Why have I not posted here yet?
Anyway, here are my favorites, highest to lowest.
The Chamber of the Sacred Markers - I just always liked that objective, plus the backdrop was much better than any other center room.
The Pit of the Pendulum - Finally a Pit room where ALL the unlocked doors open. Plus a pretty cool objective.
The Jester's Court - Same as other people: I thought the post-objective lighting was awesome.
The Shriiiiine of the Silver Monkey - The classic icon of the show. It annoyed me how much people sucked at it though.
The Room of the Ancient Warriors - I thought it was a cool design for the room. And the occasional shitting of the pants from Temple Guard encounters.
The Crypt - What better way is there to start a temple run than by running into a foggy room full of skeletons screaming at you to "GET OUT OF HERE"?
The King's Storeroom (Season 3) - Smashing pots is always fun. I prefer the Season 3 version because it was less spacious, and there was an area on the floor to break the pots so the pieces wouldn't fly everywhere.
The Laser Light Room - The bottom rooms weren't my favorites, but this one I liked.
The Room of the Secret Password (Season 3) - I liked the objective, plus in Season 3 they had to do it no matter which way they came in.