And the winner is the SHRIIIIIIIIIIIIIIIIINE OF THE SILVER MONKEY!
No surprise at all, it was the one room that appeared in every single episode on the show. This room was iconic and a staple part of the show. Even if the show went on for 10 more years, I would expect to still see this room in the temple. I don't think it was even downvoted once either.

#1: Shrine of the Silver Monkey
#2: King's Storeroom - Season 2
#3: Dark Forest
#4: Room of the Secret Password
#5: Jester's Court
#6: Observatory - Stone Column
#7: Laser Light Room
#8: Bamboo Forest
#9: Chamber of the Sacred Markers
#10: King's Storeroom - Season 3